This past month, Snowdrop DNA project students have had the opportunity to work with new technology and learn new techniques that would not have been taught in normal lessons. This has included vortexing, centrifugation, micropipetting and agarose gel electrophoresis.
Students were able to practice using micropipettes to extract their supernatant, the liquid that remains after a solid has been separated. This skill would also come in handy during their extraction of the chloroplast DNA.
Students also explored Gel electrophoresis to separate mixtures of DNA. Along with this, they developed the use of centrifuges and a vortex to extract chloroplasts and separate them from other parts of the plant cells. Analysis of these chloroplasts was undertaken using microscopy to see what they looked like and show that they had been successfully extracted. This was also contrasted to spinach chloroplast to see if there was any large difference.
Having not taken biology A-level, I was really happy to get the opportunity to still explore the subject and work in the department and learn new skills with different technology in contrast to my other subjectsGeorgia Lovatt
Although many of these students had never come across these techniques, they were all happy to learn. Making mistakes was inevitable, however, smiles were on all the students’ faces by the end of the lessons.
We got hands-on with micropipetting and gel electrophoresis, developing skills that are highly relevant for STEM careers in molecular biology. Students showed excellent accuracy and got amazing results on their gels. This was an amazing opportunity for students working on novel research as part of the snowdrop DNA project”Karen Stevens
We look forward to seeing our students accomplish more with their project. Great work from the Snowdrop Students!